TopCount Topics Application Notes Table of Contents

TopCount Topics Application Notes Table of Contents
001.	Counting Non-Aqueous Samples in the TopCount Microplate Scintillation Counter Solid phase IRMA, efficiencies in PicoPlates, organic samples
002.	Solid Scintillation Counting LumaPlates™ : efficiencies, crosstalk, color quench correction, ⁵¹Cr and ¹²⁵I
003.	Theory of TopCount Operation Basic scintillation theory, single-PMT time resolved pulse discrimination, efficiency and background data, fast vs. slow scintillators
004.	Scintillation Proximity Assay on the TopCount Microplate Scintillation Counter SPA: results from RIA kits on TopCount^® vs. LSC; color quench correction
005.	Counting Aqueous Samples With the TopCount Microplate Scintillation Counter Aqueous counting: efficiencies, chemical quench correction, DPM recoveries, sample load vs. efficiency in MicroScint cocktails
006.	TopCount Tandem Processing Using Automated Spreadsheets Spreadsheet macros and tandem processing for Lotus 123 and Quattro Pro
007.	Solid-Phase RIA in Microplates RIA and IRMA in-plate binding, streptavidin, TopCount^® vs. Gamma Counter, polystyrene resistance to MicroScint cocktails
008.	Counting ⁵¹Cr Released in Cytotoxicity Assays Cytotoxicity (CML) assays, ⁵¹Cr, TopCount^® vs. Gamma results, LumaPlates
009.	Cell Proliferation Assays ³H thymidine uptake assay, TopCount^® vs. LSC, UniFilter™, MicroMate 196™
010.	Two Methods of Harvesting Receptor Binding Assays Ligand-receptor binding, MicroMate 196™, Unifilter™, Brandel harvester
011.	Direct Counting of Millipore^® MultiScreen^® Filtration Plates Cellular receptor binding, cell proliferation, enzyme inhibition and kinase assays, counting efficiencies
012	Biological Applications of Microplate Scintillation Counting General overview of cell proliferation, receptor binding, and in-plate binding assays, comparisons with LSC
013.	Crosstalk Plate layout, ³H, ¹²⁵I, ¹⁴C, ³²P, chemiluminescence in a black plate
014.	The Measurement of Luminescence Signal linearity and detection limits on TopCount, Promega (Luciferase), Lumigen (LumiPhos), Amersham (ECL, luminol), etc.
015.	Quench and Quench Correction Basic theory of quench and correction, protocols for chemical and color quench correction curves on TopCount using UniFilter, LumaPlate, PicoPlate, OptiPlate
016.	ViewPlates : Methodology for Counting Isotopic and Luminescent Labels for Use in Adherent Cell Assays Summary table of optimal counting parameters for ViewPlates on TopCount, efficiency and % optical crosstalk, ³ H, ¹⁴ C, ⁴⁵ Ca, ³² P, ¹²⁵ I, luminescence
017.	Reporter Gene Assays Reporter gene, enzyme activity measurement assays, decay macro for short lived glow-type signals like Promega Luciferase, LumiGal
018.	Counting Radioisotopic and Luminescent Labels on Filters and Membranes With the FlexiFilter Plate FlexiFilter for use in radioactive and non-radioactive detection, recommended try and collimator combinations, kinase assay, dot blot, cell proliferation
019.	Quench Correction in Scintillation Proximity Assays Protocol for color quench correction for SPA
020.	Nucleic Acid Quantification by Chemiluminescence Assay of Polymerase Chain Reaction Products Quantitative PCR, streptavidin coated plates, xanthine oxidase/ luminol
021.	High Throughput Screening for cAMP Formation by Scintillation Proximity Radioimmunoassay Detecting cAMP by competitive SPA, 12-detectors, MultiPROBE
022.	Determination of Neutrophil Activation by Chemiluminescence Using the Packard TopCount Microplate Scintillation and Luminescence Counter Low activity luminescent signal with 15 minute signal stability, LitePlate
023.	High Throughput Luminescence Assays Using Six Detectors Multiple detector uniformity and linearity, LucLite and CytoLite
024.	Cerenkov Counting Performance on the TopCount Microplate Scintillation and Luminescence Counter Counting performance on TopCount for linearity, volume effects, efficiency, background, and crosstalk, liquid and filters, microccentrifuge tube carriers
025.	Automated Recording of Luciferase-Reported Gene Transcription in Living Seedlings and Fruit Flies Circadian rhythm, reporter gene assays, luciferase
026.	Implementation of a Robotics system for High Throughput Screening Utilizing Glow Luminescence Cell based assay and compound distribution, MultiPROBE and Zymark integration
027.	Use of TopCount for Radioactivity Determination in Wipe Test Use of the PicoPlate-24 and filter paper for wipe tests
028.	Dual Label DPM on the TopCount Constant quench dual label DPM using calculations in custom cells
029.	High Efficiency Count Mode for SPA and Cytostar-T Assays HECM using single pulse and lower discrimination adjustments
030.	Optimization of ³³P Scintillation Proximity Assays Using Cesium Chloride Bead Suspension Flotation method for SPA, reduces quench, higher efficiency
031.	³³P Counting Performance on the TopCount and TopCount NXT Microplate Scintillation and Luminescence Counters Proves counting of ³³P on the TopCount, and discusses optimization
032.	Radioimmunoprecipitation Assay for Antibodies to Glutamic Acid Decarboxylase and Other Autoantigens in Microplates Unique application for UniFilter
AN4001-TC.	This application note was replaced with AN4003-TC.
AN4002-TC.	Calibration, Normalization and IPA of the TopCount NXT Serves as a supplement to the TopCount NXT Operation Manual
AN4003-TC.	Free Fat Cell Assay Validates MicroScint E and TopCount for in-plate lipid extraction and counting
AN004-TC.	Using the TopCount Microplate Scintillation and Luminescence Counter and Deep-Well LumaPlate Microplates in Combination with Micro Separation Techniques for Metabolic Studies Proves that TopCount/ LumaPlate is most sensitive for HPLC fraction counting

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