TopCount Topics Application Notes - PerkinElmer Japan

TopCount Topics Application Notes Table of Contents

TopCount Topics Application Notes Table of Contents
001. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation Counter
Solid phase IRMA, efficiencies in PicoPlates, organic samples
002. Solid Scintillation Counting
LumaPlates™ : efficiencies, crosstalk, color quench correction, 51Cr and 125I
003. Theory of TopCount Operation
Basic scintillation theory, single-PMT time resolved pulse discrimination, efficiency and background data, fast vs. slow scintillators
004. Scintillation Proximity Assay on the TopCount Microplate Scintillation Counter
SPA: results from RIA kits on TopCount® vs. LSC; color quench correction
005. Counting Aqueous Samples With the TopCount Microplate Scintillation Counter
Aqueous counting: efficiencies, chemical quench correction, DPM recoveries, sample load vs. efficiency in MicroScint cocktails
006. TopCount Tandem Processing Using Automated Spreadsheets
Spreadsheet macros and tandem processing for Lotus 123 and Quattro Pro
007. Solid-Phase RIA in Microplates
RIA and IRMA in-plate binding, streptavidin, TopCount® vs. Gamma Counter, polystyrene resistance to MicroScint cocktails
008. Counting 51Cr Released in Cytotoxicity Assays
Cytotoxicity (CML) assays, 51Cr, TopCount® vs. Gamma results, LumaPlates
009. Cell Proliferation Assays
3H thymidine uptake assay, TopCount® vs. LSC, UniFilter™, MicroMate 196™
010. Two Methods of Harvesting Receptor Binding Assays
Ligand-receptor binding, MicroMate 196™, Unifilter™, Brandel harvester
011. Direct Counting of Millipore® MultiScreen® Filtration Plates
Cellular receptor binding, cell proliferation, enzyme inhibition and kinase assays, counting efficiencies
012 Biological Applications of Microplate Scintillation Counting
General overview of cell proliferation, receptor binding, and in-plate binding assays, comparisons with LSC
013. Crosstalk
Plate layout, 3H, 125I, 14C, 32P, chemiluminescence in a black plate
014. The Measurement of Luminescence
Signal linearity and detection limits on TopCount, Promega (Luciferase), Lumigen (LumiPhos), Amersham (ECL, luminol), etc.
015. Quench and Quench Correction
Basic theory of quench and correction, protocols for chemical and color quench correction curves on TopCount using UniFilter, LumaPlate, PicoPlate, OptiPlate
016. ViewPlates : Methodology for Counting Isotopic and Luminescent Labels for Use in Adherent Cell Assays
Summary table of optimal counting parameters for ViewPlates on TopCount, efficiency and % optical crosstalk, 3 H, 14 C, 45 Ca, 32 P, 125 I, luminescence
017. Reporter Gene Assays
Reporter gene, enzyme activity measurement assays, decay macro for short lived glow-type signals like Promega Luciferase, LumiGal
018. Counting Radioisotopic and Luminescent Labels on Filters and Membranes With the FlexiFilter Plate
FlexiFilter for use in radioactive and non-radioactive detection, recommended try and collimator combinations, kinase assay, dot blot, cell proliferation
019. Quench Correction in Scintillation Proximity Assays
Protocol for color quench correction for SPA
020. Nucleic Acid Quantification by Chemiluminescence Assay of Polymerase Chain Reaction Products
Quantitative PCR, streptavidin coated plates, xanthine oxidase/ luminol
021. High Throughput Screening for cAMP Formation by Scintillation Proximity Radioimmunoassay
Detecting cAMP by competitive SPA, 12-detectors, MultiPROBE
022. Determination of Neutrophil Activation by Chemiluminescence Using the Packard TopCount Microplate Scintillation and Luminescence Counter
Low activity luminescent signal with 15 minute signal stability, LitePlate
023. High Throughput Luminescence Assays Using Six Detectors
Multiple detector uniformity and linearity, LucLite and CytoLite
024. Cerenkov Counting Performance on the TopCount Microplate Scintillation and Luminescence Counter
Counting performance on TopCount for linearity, volume effects, efficiency, background, and crosstalk, liquid and filters, microccentrifuge tube carriers
025. Automated Recording of Luciferase-Reported Gene Transcription in Living Seedlings and Fruit Flies
Circadian rhythm, reporter gene assays, luciferase
026. Implementation of a Robotics system for High Throughput Screening Utilizing Glow Luminescence
Cell based assay and compound distribution, MultiPROBE and Zymark integration
027. Use of TopCount for Radioactivity Determination in Wipe Test
Use of the PicoPlate-24 and filter paper for wipe tests
028. Dual Label DPM on the TopCount
Constant quench dual label DPM using calculations in custom cells
029. High Efficiency Count Mode for SPA and Cytostar-T Assays
HECM using single pulse and lower discrimination adjustments
030. Optimization of 33P Scintillation Proximity Assays Using Cesium Chloride Bead Suspension
Flotation method for SPA, reduces quench, higher efficiency
031. 33P Counting Performance on the TopCount and TopCount NXT Microplate Scintillation and Luminescence Counters
Proves counting of 33P on the TopCount, and discusses optimization
032. Radioimmunoprecipitation Assay for Antibodies to Glutamic Acid Decarboxylase and Other Autoantigens in Microplates
Unique application for UniFilter
AN4001-TC. This application note was replaced with AN4003-TC.
AN4002-TC. Calibration, Normalization and IPA of the TopCount NXT
Serves as a supplement to the TopCount NXT Operation Manual
AN4003-TC. Free Fat Cell Assay
Validates MicroScint E and TopCount for in-plate lipid extraction and counting
AN004-TC. Using the TopCount Microplate Scintillation and Luminescence Counter and Deep-Well LumaPlate Microplates in Combination with Micro Separation Techniques for Metabolic Studies
Proves that TopCount/ LumaPlate is most sensitive for HPLC fraction counting