PhenoVue Fluor - Goat anti-Rabbit (H+L) Antibody Conjugates - PerkinElmer Japan

PhenoVue Fluor - Goat anti-Rabbit (H+L) Antibody Conjugates

Goat anti-Rabbit IgG (H+L) antibodies は、当社の高輝度 PhenoVue™ 蛍光色素で標識された二次抗体です。
PhenoVue Fluor dyes - Goat anti-Rabbit IgG (H+L) anti-Rabbit IgG (H+L) antibody Cross-adsorbed は、アフィニティー精製されており、ウサギ IgG を認識し、ラットやハムスターなどの他の種との交差反応性を示すことがあります。
PhenoVue PhenoVue Fluor dyes - Goat anti-Rabbit IgG (H+L) antibodies Highly Cross-adsorbed は、アフィニティ精製後、ラット、マウス、ハムスター、ヒト、ウシ、ウマ、ニワトリの血清など様々なIgG に対して吸着処理を行い、交差反応性を最小限に抑えています。

 

製品名 品番 バイアル数 数量 形態 発送条件 保存*
PhenoVue Fluor 488 -
Goat anti-Rabbit antibody
Cross-adsorbed
2GXRB488C1
1 1 mg 凍結乾燥 RT 2 - 8℃
PhenoVue Fluor 555 -
Goat anti-Rabbit antibody
Cross-adsorbed
2GXRB555C1
PhenoVue Fluor 568 -
Goat anti-Rabbit antibody
Cross-adsorbed
2GXRB568C1
PhenoVue Fluor 594 -
Goat anti-Rabbit antibody
Cross-adsorbed
2GXRB594C1
PhenoVue Fluor 647 -
Goat anti-Rabbit antibody
Cross-adsorbed
2GXRB647C1
PhenoVue Fluor 488 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
2GXRB488H1
1 1 mg 凍結乾燥 RT 2 - 8℃
PhenoVue Fluor 555 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
2GXRB555H1
PhenoVue Fluor 568 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
2GXRB568H1
PhenoVue Fluor 594 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
2GXRB594H1
PhenoVue Fluor 647 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
2GXRB647H1

* 製品の安定性は、推奨通りに遮光・保存されていれば、分析証明書に記載されている有効期限まで保証されています。一度再構成された原液は、≤-16℃以下で保存保存します。

 

相当プレート数

製品名 推奨使用濃度
96-well
(100µL-300µL/Well)
384-well
(25µL-90µL/Well)
1536-well
(4µL-12µL/Well)
PhenoVue Fluor 488 -
Goat anti-Rabbit antibody
Cross-adsorbed
1.5 µg/mL
(10 nM)
約 25 - 70 約 20 - 70 約 35 - 90
PhenoVue Fluor 555 -
Goat anti-Rabbit antibody
Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Rabbit antibody
Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Rabbit antibody
Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Rabbit antibody
Cross-adsorbed
PhenoVue Fluor 488 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
1.5 µg/mL
(10 nM)
約 25 - 70 約 20 - 70 約 35 - 90
PhenoVue Fluor 555 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed

 

推奨の再構成​

製品名 分子量 推奨保存濃度 作業濃度範囲*
PhenoVue Fluor 488 -
Goat anti-Rabbit antibody
Cross-adsorbed
150000 g.mol-1 1 mL の ddH2O を使用して再構成すると、1 mg/mL (6.66 µM) になります
0.1 µg/mL - 10 µg/mL
(0.66 nM - 66.6 nM)
PhenoVue Fluor 555 -
Goat anti-Rabbit antibody
Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Rabbit antibody
Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Rabbit antibody
Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Rabbit antibody
Cross-adsorbed
PhenoVue Fluor 488 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
150000 g.mol-1 1 mL の ddH2O を使用して再構成すると、1 mg/mL (6.66 µM) になります
0.1 µg/mL - 10 µg/mL
(0.66 nM - 66.6 nM)
PhenoVue Fluor 555 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Rabbit antibody
Highly Cross-adsorbed

* PBS で希釈できます。

 

スペクトルと光物性

製品名 最大励起
波長 (nm)
最大発光
波長 (nm)
共通フィル
ターセット
量子収量
(Φ)
イプシロン*
(ε in M-1.cm-1
at λ max)
明るさ
(Φ x ε)
PhenoVue Fluor 488 495 520 FITC 92 % 73000 65320
PhenoVue Fluor 555 555 570 Cy3 10 % 155000 15500
PhenoVue Fluor 568 578 603 Texas-Red 69 % 88000 60720
PhenoVue Fluoe 594 590 617 Texas-Red 66 % 92000 60720
PhenoVue Fluor 647 650 670 Cy5 30 % 240000 72000

* In Methanol

 

交差反応性

製品名 Across Species
PhenoVue Fluor 488 - Goat anti-Rabbit antibody Cross-adsorbed Guinea Pig : 10%
Human IgG : 6.8%
PhenoVue Fluor 555 - Goat anti-Rabbit antibody Cross-adsorbed
PhenoVue Fluor 568 - Goat anti-Rabbit antibody Cross-adsorbed
PhenoVue Fluor 594 - Goat anti-Rabbit antibody Cross-adsorbed
PhenoVue Fluor 647 - Goat anti-Rabbit antibody Cross-adsorbed
PhenoVue Fluor 488 - Goat anti-Rabbit antibody Highly Cross-adsorbed
Guinea Pig : 8%
Human IgG : 0.6%
PhenoVue Fluor 555 - Goat anti-Rabbit antibody Highly Cross-adsorbed
PhenoVue Fluor 568 - Goat anti-Rabbit antibody Highly Cross-adsorbed
PhenoVue Fluor 594 - Goat anti-Rabbit antibody Highly Cross-adsorbed
PhenoVue Fluor 647 - Goat anti-Rabbit antibody Highly Cross-adsorbed

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