PhenoVue Fluor - Goat anti-Mouse IgG (H+L) Antibody Conjugates - PerkinElmer Japan

PhenoVue Fluor - Goat anti-Mouse IgG (H+L) Antibody Conjugates

Goat anti-mouse IgG (H+L) antibodies は、当社の高輝度 PhenoVue™ 蛍光色素で標識された二次抗体です。
PhenoVue Fluor dyes Goat anti-mouse IgG (H+L) antibody, Cross-adsorbed は、アフィニティ精製されており、異なるマウス IgG アイソタイプを認識し、ラットやハムスターなどの他の種との交差反応性を示すことがあります。
PhenoVue Fluor dye Goat anti-mouse IgG (H+L) antibody, Highly Cross-adsorbed は、アフィニティ精製後、ラット、ハムスター、ウシ、ウマ、ヒト、ウサギ、またはニワトリなどの様々な IgG に対して吸着処理を行い、交差反応性を最小限に抑えています。

 

製品名 品番 バイアル数 数量 形態 発送条件 保存*
PhenoVue Fluor 488 -
Goat anti-Mouse antibody
Cross-adsorbed
2GXM488C1
1 1 mg 凍結乾燥 RT 2 - 8℃
PhenoVue Fluor 555 -
Goat anti-Mouse antibody
Cross-adsorbed
2GXM555C1
PhenoVue Fluor 568 -
Goat anti-Mouse antibody
Cross-adsorbed
2GXM568C1
PhenoVue Fluor 594 -
Goat anti-Mouse antibody
Cross-adsorbed
2GXM594C1
PhenoVue Fluor 647 -
Goat anti-Mouse antibody
Cross-adsorbed
2GXM647C1
PhenoVue Fluor 488 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
2GXM488H1
1 1 mg 凍結乾燥 RT 2 - 8℃
PhenoVue Fluor 555 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
2GXM555H1
PhenoVue Fluor 568 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
2GXM568H1
PhenoVue Fluor 594 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
2GXM594H1
PhenoVue Fluor 647 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
2GXM647H1

* 製品の安定性は、推奨通りに遮光・保存されている場合、分析証明書に記載されている有効期限まで保証されています。一度再構成した原液は、-16℃以下で保存します。

 

相当プレート数

製品名 推奨使用濃度
96-well
(100µL-300µL/Well)
384-well
(25µL-90µL/Well)
1536-well
(4µL-12µL/Well)
PhenoVue Fluor 488 -
Goat anti-Mouse antibody
Cross-adsorbed
1.5 µg/mL
(10 nM)
約 25 - 70 約 20 - 70 約 35 - 90
PhenoVue Fluor 555 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 488 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
1.5 µg/mL
(10 nM)
約 25 - 70 約 20 - 70 約 35 - 90
PhenoVue Fluor 555 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Mouse antibody
Highly Cross-adsorbed

 

推奨の再構成​

製品名 分子量 推奨保存濃度 作業濃度範囲*
PhenoVue Fluor 488 -
Goat anti-Mouse antibody
Cross-adsorbed
150000 g.mol-1 1 mL の ddH2O を使用して再構成すると、1 mg/mL (6.66 µM) になります
0.1 µg/mL - 10 µg/mL
(0.66 nM - 66.6 nM)
PhenoVue Fluor 555 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 488 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
150000 g.mol-1 1 mL の ddH2O を使用して再構成すると、1 mg/mL (6.66 µM) になります
0.1 µg/mL - 10 µg/mL
(0.66 nM - 66.6 nM)
PhenoVue Fluor 555 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Mouse antibody
Highly Cross-adsorbed

* PBS で希釈できます。

 

スペクトルと光物性

製品名 最大励起
波長 (nm)
最大発光
波長 (nm)
共通フィル
ターセット
量子収量
(Φ)
イプシロン*
(ε in M-1.cm-1 at λ max)
明るさ
(Φ x ε)
PhenoVue Fluor 488 495 520 FITC 92 % 73000 65320
PhenoVue Fluor 555 555 570 Cy3 10 % 155000 15500
PhenoVue Fluor 568 578 603 Texas-Red 69 % 88000 60720
PhenoVue Fluoe 594 590 617 Texas-Red 66 % 92000 60720
PhenoVue Fluor 647 650 670 Cy5 30 % 140000 72000

* In Methanol

 

交差反応性

製品名 Across Species* (>10%)
Across IgG isotypes
PhenoVue Fluor 488 -
Goat anti-Mouse antibody
Cross-adsorbed
Rat: 44%
Guinea Pig: 16%
Hamster: 50%
Horse: 10%
Cross-reactivity with mouse:
IgG1
IgG2a
IgG2b
IgG2C
IgG3
PhenoVue Fluor 555 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Mouse antibody
Cross-adsorbed
PhenoVue Fluor 488 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
None Cross-reactivity with mouse:
IgG1
IgG2a
IgG2b
IgG2C
IgG3
PhenoVue Fluor 555 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 568 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 594 -
Goat anti-Mouse antibody
Highly Cross-adsorbed
PhenoVue Fluor 647 -
Goat anti-Mouse antibody
Highly Cross-adsorbed

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