Opalキット | TSA 免疫組織化学染色・in situ Hybridization増感システム - PerkinElmer Japan

Opalキット

 

Increase Imaging Detection 100X with Tyramide Signal Amplification (TSA)!

Amplify Your IHC, IF or ISH Signal with TSA Reagent Solutions ...

Why Multiplex?!

Why Multiplex?

Learn how TSA dramatically expands options for Multiplex detection ... View Rapid Learning Presentation

Signal Amplification for Immunohistochemistry

Signal Amplification for Immunohistochemistry

Improve the sensitivity of detection by 100-fold or more ... View Rapid Learning Presentation

Signal Amplification for In Situ Hybridization

Signal Amplification for In Situ Hybridization

Get higher sensitivity with minimal change to your existing protocol ... View Rapid Learning Presentation

TSA Published Papers

TSA Published Papers

View peer-reviewed scientific literature published by researchers using TSA solutions ... Read More

  • Increase Imaging Detection 100X with Tyramide Signal Amplification (TSA)!
  • Why Multiplex?
  • Signal Amplification for Immunohistochemistry
  • Signal Amplification for In Situ Hybridization
  • TSA Published Papers
Opal キット

FFPE組織切片において、複数の抗体を使った多重蛍光検出を行うTSA Plusシステムです。TSA検出の後、抗原賦活化溶液中で電子レンジ加熱を行うことで、一次抗体やHRP標識二次抗体を取り除くプロトコールを採用していますので、同じ動物種の一次抗体であっても多重検出を行うことが可能です。
組織染色のサイトメトリー解析に必須の染色技術です。パーキンエルマーのイメージング技術と合わせたPhenoptics research solutionによる先進のがん微小環境研究・がん免疫研究が注目されています。

Phenoptics research solutionは2015年 The Scientist誌のTop10 innovationsに選ばれました。



Opal kitによる乳がん組織FFPE組織切片のバイオマーカー検出

 
ER+, PR+, HER2+, Ki-67- ER+, PR+, HER2+, Ki-67+

Opal Staining in Breast Cancer. A 4-plex IHC using TSA Plus to detect Estrogen Receptor (green), Progesteron Receptor (yellow), HER2 (red), Ki-67 (purple) and DAPI (blue). Imaged with Vectra 

注意 Opalキット製品受領後はOpal Reagentを-20℃で保管してください。DMSO溶解後は4℃冷蔵保管です。

 

Opal マニュアルキット

製品名 数量 品番
Opal 4-Color Manual IHC Kit 50 slides NEL810001KT
Opal 7-Color Manual IHC Kit 50 slides NEL811001KT

NEL810001KT構成

  • 1X Plus Amplification Diluent (1 X 50 mL)
  • Opal 520 Reagent
  • Opal 570 Reagent
  • Opal 690 Reagent
  • DMSO ( 1 X 500 µL)
  • Spectral DAPI solution (1 X 1.5 mL)
  • Opal polymer HRP Ms+Rb ( 1 X 50mL)
  • Blocking/Ab Diluent (1 X 100 mL)
  • 10X AR6 buffer (2 X  250ml)

NEL811001KT構成

  • 1X Plus Amplification Diluent (2 X 50 mL)
  • Opal 520 Reagent
  • Opal 540 Reagent
  • Opal 570 Reagent
  • Opal 620 Reagent
  • Opal 650 Reagent
  • Opal 690 Reagent
  • DMSO ( 1 X 500 µL)
  • Spectral DAPI solution (1 X 1.5 mL )
  • Opal polymer HRP Ms+Rb ( 2 X 50mL)
  • Blocking/Ab Diluent (1 X 100 mL)
  • 10X antigen retrieval buffer pH6 (AR6) (4 X  250ml)

 

Opal 自動染色機用キット

製品名 数量 品番
Opal 4-Color Automation IHC Kit* 50 slides NEL800001KT
Opal 7-Color Automation IHC Kit* 50 slides NEL801001KT

* Leica Biosystems BOND RX System向け

NEL800001KT構成

  • 1X Plus Amplification Diluent (1 X 50 mL)
  • Opal 520 Reagent
  • Opal 570 Reagent
  • Opal 690 Reagent
  • DMSO (1 X 500 μL)
  • Spectral DAPI solution (1 X 1.5 mL)
  • Blocking/Ab Diluent (1 X 100 mL)
  • Opal Polymer HRP Ms + Rb (1 x 50 mL)

 

NEL801001KT構成

  • 1X Plus Amplification Diluent (2 X 50 mL)
  • Opal 520 Reagent
  • Opal 540 Reagent
  • Opal 570 Reagent
  • Opal 620 Reagent
  • Opal 650 Reagent
  • Opal 690 Reagent
  • DMSO ( 1 X 500 µL)
  • Spectral DAPI solution (1 X 1.5 mL )
  • Opal polymer HRP Ms+Rb ( 2 X 50mL)
  • Blocking/Ab Diluent (1 X 100 mL)

 

関連製品

Opal Multiplex IHC Detection Kits

製品名 数量 品番
Opal 4 Lymphocyte Kit 50 slides OP4LY1001KT
Opal 7 Immunology Discovery Kit 50 slides OP7DS1001KT
Opal 7 Tumor Infiltrating Lymphocyte Kit 50 slides OP7TL1001KT
Opal 7 Solid Tumor Immunology Kit 50 slides OP7TL2001KT

 

Opal Reagents

Opal各試薬はDMSOとのセット単品での購入が可能です。

製品名 品番
Opal520 Reagent, powder FP1487001KT
Opal540 Reagent, powder FP1494001KT
Opal570 Reagent, powder FP1488001KT
Opal620 Reagent, powder FP1495001KT
Opal650 Reagent, powder FP1496001KT
Opal690 Reagent, powder FP1497001KT
Spectral DAPI FP1490001KT

 

Ancillary

製品名 品番
1X Plus Amplification Diluent 1 X 50 mL FP1498A
AR6 buffer (10X) 4 x 250 mL AR6001KT
AR6 buffer (10X) 250 mL AR600250ML
AR9 buffer (10X) 4 x 250 mL AR9001KT
AR9 buffer (10X) 250 mL AR900250ML
Antibody Diluent / Block 100 mL ARD1001A
Opal Polymer HRP Ms + Rb 50 mL ARH1001A

保存:4℃(遮光下)

 

文献

Stack, E. C. et al. Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods (2014),

Tornehave, D. et al. Microwaving for double indirect immunofluorescence with primary antibodies from the same species and for staining of mouse tissues with mouse monoclonal antibodies. Histochem. Cell Biol. 113: 19-23 (2000)

Tóth, Z.E. and Mezey, É. Simultaneous Visualization of Multiple Antigens with Tyramide Signal Amplification using Antibodies from the same Species. J Histochem Cytochem. 55: 545-554 (2007).