TSA関連リファレンスガイド

Berger, U.V. and Hediger, M.A. Comparative analysis of glutamate transporter expression in rat brain using differential double in situ hybridization. Anat. Embryol. 198:13-30 (1998).

Broberger, C.,et al. Subtypes Y1 and Y2 of the neuropeptide Y receptor are respectively expressed in pro-opiomelanocortin- and Neuropeptide -Y-containing neurons of the rat hypothalamic arcuate nucleus. Neuroendocrinology 66:393-408 (1997).

Brouns, I., et al. Triple Immunofluorescence Staining with Antibodies Raised in the Same Species to Study the Complex Innervation Pattern of Intrapulmonary Chemoreceptors. J.Histochem. Cytochem. 50(4):575-582, 2002.

Dunn-Meynell, A.A., et al. Distribution and phenotype of neurons containing the ATP-sensitive K+ channel in rat brain. Brain. Res. 814:41-54 (1998)

Speel, E.J.M., et al. Sensitive Multicolor Fluorescence In Situ Hybridization Using Catalyzed Reporter Deposition (CARD) Amplification. J. Histochem. Cytochem. 45(10):1439-1446, 1997.

Hunyady, B., et al. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostainings. J. Histochem. Cytochem. 44:1353-1362 (1996).

Jackerott, M. and Larsson, L-I. Immunocytochemical localization of the NPY/PYY Y1 receptor in enteric neurons, endothelial cells, and endocrine-like cells of the rat intestinal tract. J. of Histochem.Cytochem. 45:1643-1650 (1997).

Mastroberardino, L., B. et al. Amino acid transport by heterodimers of 4F2hc/CD98 and members of a permease family. Nature 395:288-291 (1998).

Moen, P.T. Multi-target detection using tyramid signal amplification : Fluorescence in situ DNA and unclear RNA hybridization using TSA.

Paratore, C., et al. Embryonic gene expression resolved at the cellular level by fluorescence in situ hybridization. Histochem. Cell Biol. 111:435-443 (1999).

Portela-Gomes, G., et al. Co-localization of synaptophysin with different neuroendocrine hormones in the human gastrointestinal tract. Histochem. Cell Biol. 111:49-54 (1999).

Punnonena, E-L., et al. Ultrastructural Localization of β-Actin and Amphoterin mRNA in Cultured Cells: Application of Tyramide Signal Amplification and Comparison of Detection Methods. J.Histochem. Cytochem. 47(1):99-112, 1999.

Shindler, K.S. and Roth, K.A. Double immunofluorescent staining using two unconjugated primary antisera raised to the same species. J.Histochem. Cytochem. 44(11):1331-1335 (1996).

Swindle, C.S., et al. Human papillomavirus DNA replication compartments in a transient DNA replication system. J. Virology 73:1001-1009 (1999).

Villeneuve, P., et al. Immunohistochemical distribution of the prohormone convertase PC5-A in rat brain. Neuroscience 92:641-654 (1999).

Wang, G., et al. Tyramide signal amplification method in multiple-label immunofluorescence confocal microscopy. Methods 18:459-464 (1999).

Xu, Z.-Q.D., et al. Galanin-5-hydroxytryptamine interactions: Electrophysiological, immunohistochemical and in situ hybridization studies on rat dorsal raphe neurons with a note on galanin R1and R2 receptors. Neuroscience 87:79-94 (1998).

Zaidi, A.U., et al. Dual Fluorescent In Situ Hybridization and Immunohistochemical Detection with Tyramide Signal Amplification. J.Histochem. Cytochem. 48(10):1369–1375, 2000.

Adler, K., et al. High Sensitivity detection of HPV-16 in SiHa and CaSki cells using FISH enhanced by TSA. Histochem. Cell Biol. 108:321-324 (1997).

Flore, O. et al. Transformation of primary human endothelial cells by Kaposi's sarcoma-associated herpesvirus. Nature 394:588-592 (1998).

Komminoth, P. and Werner, M. Target and signal amplification: Approaches to increase the sensitivity of in situ hybridization. Histochem. Cell Biol. 108:325-333 (1997).

Strappe P.M., et al. In situ polymerase chain reaction amplification of HIV-1 DNA in brain tissue. J. Virol. Methods 70(2):119-127 (1998).

Wiedorn, K.H., et al. Comparison of in-situ hybridization, direct and indirect in-situ PCR as well as tyramide signal amplification for detection of HPV. Histochem.Cell Biol. 111:89-95 (1999).

Zehbe, I. et al. Sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-Nanogold, and silver acetate autometallography: detection of single-copy human papillomavirus. Am. J. Path. 150:1553-1561 (1997).

Aaltonen, J. et al. High-resolution physical and transcriptional mapping of the autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy locus on chromosome 21q22.3 by FISH. Genome Res. 7:820-829 (1997).

Ballestas, M.E. et al. Efficient persistence of extrachromosomal KSHV DNA mediated by latency-associated nuclear antigen. Science 284:641-644 (1999).

Deichmann, M. et al. Ultra-sensitive FISH is a useful tool for studying chronic HIV-1 infection. J. Virol. Methods 65:19-25 (1997).

Evans, M.F. et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens. BMC Clinical Pathology 3(2):1-17 (2003).

Horelli-Kuitunen, N. et al. Mapping ESTs by FIBER-FISH. Genome Methods 9:62-71 (1999).

Khrustaleva, L.I. and Kik, C. Localization of single-copy T-DNA insertion in transgenic shallots (Allium cepa) by using ultra-sensitive FISH with tyramide signal amplification. The Plant Journal 25(6):699-707 (2001).

Klockars, T. et al. The visual assignment of genes by Fiber-FISH: BTF3 protein homologue gene (BTF3) and a novel pseudogene of human RNA helicase A (DDX9P) on 13q22. Genomics 44:355-357 (1997).

Macechko, P.T. et al. Comparison of immunologic amplification vs enzymatic deposition of fluorochrome-conjugated tyramide as detection systems for FISH. J. Histochem. Cytochem. 45:359-363 (1997).

McKay, J.A. et al. Amplification of fluorescent in situ hybridization signals in formalin fixed paraffin wax embedded sections of colon tumor using biotinylated tyramide. Mol. Pathol. 50(6):322-325 (1997).

Raap, A.K. et al. Ultra-sensitive FISH using peroxidase-mediated deposition of biotin- or fluorochrome tyramides. Hum. Molec. Gen. 4:529-534 (1995).

Schriml, L.M. et al. Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27:608-613 (1999).

Speel, E.J.M. et al. Sensitive Multicolor Fluorescence In Situ Hybridization Using Catalyzed Reporter Deposition (CARD) Amplification. J. Histochem. Cytochem. 45(10):1439–1446 (1997).

Stephens, J.L. et al. Physical mapping of barley genes using an ultrasensitive fluorescence in situ hybridization technique. Genome 47:179–189 (2004).

van Gijlswijk, R.P.M. et al. Horseradish peroxidase-labeled oligonucleotides and fluorescent tyramides for rapid detection of chromosome-specific repeat sequences. Cytogenet Cell Genet. 75:258-262 (1996).

van Gijlswijk, R.P.M. et al. Improved localization of fluorescent tyramides for fluorescence in situ hybridization using dextran sulfate and polyvinyl alcohol. J. Histochem. Cytochem. 44:389-392 (1996).

van Gijlswijk, R.P.M. et al. Fluorochrome-labeled Tyramides: Use in Immunocytochemistry and Fluorescent In Situ Hybridization. J. Histochem. Cytochem. 45(3):375-382 (1997).

van Tine, B.A. et al. Localization of HuC (ELAVL3) to chromosome 19p13.2 by fluorescence in situ hybridization utilizing a novel tyramide labeling technique. Genomics 53:296-299 (1998).

Brend, T. and Holley, S.A., Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization. Journal of Visualized Experiments25 (2009).

Bobrow, M.N. and Moen, P.T. Jr. Chapter 8:Unit 8.9 Tyramide Signal Amplification (TSA) Systems for the Enhancement of ISH Signals. in Cytogenetics. In Current Protocols in Cytometry. Eds. John Wiley & Sons, Inc.2003

Choi C. An in situ hybridization technique to detect low-abundance slug mRNA in adherent cultured cells. Methods Mol Biol. 326:173-188 (2006).

Cheung, A.L.M. et al. Detection of human papillomavirus in cervical carcinoma: Comparison of peroxidase, Nanogold, and catalyzed reporter deposition (CARD)-Nanogold in situ hybridization. Mod. Pathol. 12:689-696 (1999).

Clay H. and Ramakrishnan L. Multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification. Zebrafish. 2(2):105-111 (2005).

Dabeva, M.D. et al. Liver regeneration and α-fetoprotein messenger RNA expression in the retrorsine model for hepatocyte transplantation. Cancer Res. 58:5825-5834 (1998).

Davidson L.A. and Keller R.E., Neural tube closure in Xenopus laevis involves medial migration, directed protrusive activity, cell intercalation and convergent extension. Development 126:4547-4556 (1999).

Evans, M.F. et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens. BMC Clinical Pathology 3:1-17 (2003).

Hougaard, D. M. et al. Non-radioactive in situ hybridization for mRNA with emphasis on the use of oligodeoxynucleotide probes. Histochem. Cell Biol. 108:335-344 (1997).

Khan, S.H. et al. In-situ hybridization localized MUC7 mucin gene expression to the mucous acinar cells of human and MUC7-transgenic mouse salivary glands. Glycoconjugate J. 15:1125-1132 (1998).

King, G. et al. Detection of immunoglobulin light chain mRNA by in situ hybridisation using biotinylated tyramine signal amplification. Mol. Path. 52:47-50 (1999).

Lécuyer. E. et al. Fluorescent in situ hybridization protocols in Drosophila embryos and tissues.
Also refer to The Krause Lab

Mariëtte, P.C. et al. Sensitive mRNA Detection by Fluorescence In Situ Hybridization Using Horseradish Peroxidase-labeled Oligodeoxynucleotides and Tyramide Signal Amplification. J. Histochem. Cytochem. 46(11):1249-1259 (1998).

McKay, J.A. et al. Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol. Path. 50:322-325 (1997).

Moorman, A.F.M. et al. Sensitive Nonradioactive Detection of mRNA in Tissue Sections: Novel Application of the Whole-mount In Situ Hybridization Protocol. J. Histochem. Cytochem. 49(1):1-8 (2001).

Pringle, N.P. and Richardson, W.D. In situ hybrization protocols.

Silahtaroglu, A.N. et al. Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification. Nat. Protoc. 2(10):2520-2528 (2007).

Thompson, C.L., et al. Genomic Anatomy of the Hippocampus. Neuron 60(6):1010-1021 (2008).

van de Corput, M. et al. Sensitive mRNA detection by fluorescence in situ hybridization using horseradish peroxidase-labeled oligodeoxynucleotides and tyramide signal amplification. J. Histochem. Cytochem. 46(11):1249-1259 (1998).

Wanner, I. et al. Subcellular localization of specific mRNAs and their protein products in Purkinje cells by combined fluorescence in situ hybridization and immunocytochemistry. Histochem. Cell Biol. 108:345-357 (1997).

Warren, K.C. et al. Use of Methacrylate De-embedding Protocols for In Situ Hybridization on Semithin Plastic Sections with Multiple Detection Strategies. Journal of Histo. Cytochem. 46(2):149-155 (1998).

Yang, H. et al. An optimized method for in situ hybridization with signal amplification that allows the detection of rare mRNAs. J. Histochem. Cytochem. 47(4):431-445 (1999).

Welten, M.C. et al. ZebraFISH: fluorescent in situ hybridization protocol and three-dimensional imaging of gene expression patterns. Zebrafish 3(4):465-476 (2006).
Also refer to Chapter 2 ZebraFISH Fluorescent in situ hybridization protocol

Aldenborg, F. et al. Metaplastic transformation of urinary bladder epithelium. Effect on mast cell recruitment, distribution, and phenotype expression. Am. J.Pathol. 153:149-157 (1998).

Andersen-Beckh, B. et al. Polarized expression of the vasopressin V2 receptor in Madin-Darby canine kidney cells. Kidney International 56:517-527 (1999).

Balasingam, V. and V.W. Yong. Attenuation of astroglial reactivity by Interleukin-10. J. Neurosci. 16:2945-2955 (1996).

Bernardini, N. et al. The use of MAB 1977 monoclonal antibody for the immunohistochemical localization of beta 1 integrins in paraffin-embedded human kidney. Tumori 83:673-678 (1997).

Broberger, C. et al. Cocaine- and amphetamine-regulated transcript in the rat vagus nerve: A putative mediator of cholecystokinin-induced satiety. PNAS 96:13506-13511 (1999).

Büki, A. et al. Novel Application of Tyramide Signal Amplification (TSA): Ultrastructural Visualization of Double-labeled Immunofluorescent Axonal Profiles. J. Histochem. Cytochem. 48(1):153-161 (2000).

Egan, R.M., et al. Peptide-specific T cell clonal expansion in vivo following immunization in the eye, an immune-privileged site. J.Immunol. 157:2262-2271 (1996).

Grumbach, I.M. and Veh, R.W. The SA/rABC technique: A new ABC procedure for detection of antigens at increased sensitivity. J. Histochem. Cytochem. 43(1):31-37 (1995).

Hatanaka Y. et al. A simplified, sensitive immunohistochemical detection system employing signal amplification based on fluorescyl-tyramide/antifluorescein antibody reaction: its application to pathologic testing and research. Appl Immunohistochem Mol Morphol. 16(1):87-93 (2008).

Hendrikx, P.J., et al. LacZ staining in paraffin-embedded tissue sections. J. Histochem. Cytochem. 44:1323-1329 (1996).

Honda, T., et al. Co-localization of the glial cell-line derived neurotrophic factor and its functional receptor c-RET in a subpopulation of rat dorsal root ganglion neurons. Neuroscience Letters 275:45-48 (1999).

Hunyady, B., et al. Identification of endogenous peroxidase-containing cells as eosinophils in the gastrointestinal system. Histochemistry and Cell Biology 106:447-456 (1996).

Hunyady, B., et al. Immunohistochemical localization of somatostatin receptor SST2A in the rat pancreas. Endocrin. 138:2632-2635 (1997).

Isaacs, K. R., et al. A method for the rapid analysis of neuronal proportions and neurite morphology in primary cultures. Exp. Neurol. 149:464-467 (1998).

Kay-Nishiyama,C. and Watts. A.G. Dehydration modifies somal CRH immunoreactivity in the rat hypothalamus: an immunocytochemical study in the absence of colchicine. Brain Res. 822:251-255 (1999).

Kim, S.H. et al. An Improved Protocol of Biotinylated Tyramine-based Immunohistochemistry Minimizing Nonspecific Background Staining. J. Histochem. Cytochem. 51(1):129-132 (2003).

Koda, T. et al. Application of tyramide signal amplification for detection of N-glycolylneuraminic acid in human hepatocellular carcinoma. Int. J. Clin. Oncol. 8:317-321 (2003).

Kunze E. et al. Immunohistochemical staining of plastic (methyl-methacrylate)-embedded bone marrow biopsies applying the biotin-free tyramide signal amplification system. Appl Immunohistochem Mol Morphol. 16(1):76-82 (2008).

Loeb, J.A. et al. Expression patterns of transmembrane and released forms of neuregulin during spinal cord and neuromuscular synapse development. Development 126:781-791 (1999).

Loup, F. et al. A highly sensitive immunofluorescence procedure for analyzing the subcellular distribution of GABAA receptor subunits in the human brain. J. Histochem. Cytochem. 46(10):1129-1139 (1998).

Luo, J.H. et al. Detection of CD5 antigen on B cell lymphomas in fixed, paraffin embedded tissues using signal amplification by catalyzed reporter deposition. Eur. J. Histochem. 42:31-39 (1998).

Niedobitek, G. et al. Immunohistochemical detection of the Epstein-Barr Virus-encoded latent membrane protein 2A in Hodgkin's disease and infectious mononucleosis. Blood 90:1654-1672 (1997).

Pesini, P. et al. An immunohistochemical marker for Wallerian degeneration of fibers in the central and peripheral nervous system. Brain Res. 828(1-2):41-59 (1999).

Strappe P.M. et al. Enhancement of immunohistochemical detection of HIV-1 p24 antigen in brain by tyramide signal amplification. J. Virol. Methods 67:103-112 (1997).

Strous, G.J., P. et al. The ubiquitin conjugation system is required for ligand-induced endocytosis and degradation of the growth hormone receptor. The EMBO J. 15:3806-3812 (1996).

Toda, Y. at al. Application of tyramide signal amplification to immunohistochemistry: A potent method to localize antigens that are not detectable by ordinary method. Pathol. International 49:479-483 (1999).

Töpel I.I. et al. Distribution of the endothelial constitutive nitric oxide synthase in the developing rat brain: an immunohistochemical study. Brain Res. 788 (1-2):43-48 (1998).

van Gijssel H.E. et al. Immunohistochemical visualization of wild-type p53 protein in paraffin-embedded rat liver using tyramide amplification: zonal hepatic distribution of p53 protein after N-hydroxy-2-acetylaminofluorene administration. Carcinogenesis 19(1):219-222 (1998).

Wang, S. et al. Individual Subtypes of Enteroendocrine Cells in the Mouse Small Intestine Exhibit Unique Patterns of Inositol 1,4,5-trisphosphate Receptor Expression. J. Histochem. Cytochem. 52(1):53-63 (2004).

Watanabe, H. and Yamada, Y. Mice lacking link protein develop dwarfism and craniofacial abnormalitites. Nature Genetics 21:225-229 (1999).

Xu, Z.-Q. et al. Expression of galanin and a galanin receptor in several sensory systems and bone anlage of rat embryos. Proc. Natl. Acad. Sci. 93:14901-14905 (1996).

Humbel.B.M. et al. Pre-embedding immunolabeling for electron microscopy: an evaluation of permeabilization methods and markers. Microsc. Res. Tech. 42(1):43-58 (1998).

Landry, M. and Hökfelt, T. Subcellular localization of preprogalanin messenger RNA in perikarya and axons of hypothalamo-posthypophyseal magnocellular neurons: an in situ hybridization study. Neuroscience 84(3):897-912 (1998).

Lee, S-W, et al. Introduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold–Silver Staining at the Electron Microscopic Level. J. Histochem. Cytochem. 53(2):249-252 (2005).

Mayer, G. and Bendayan. M. Biotinyl-Tyramide: A novel approach for electron microscopic immunocytochemistry. J. Histochem. Cytochem. 45(11):1449-1454 (1997).

Mayer, G. and Bendayan. M. Immunogold signal amplification: Application of the CARD approach to electron microscopy. J. Histochem. Cytochem. 47(4):421-429 (1999).

Punnonen, E.-L., et al. Ultrastructural localization of β-actin and amphoterin mRNA in cultured cells: Application of tyramide signal amplification and comparison of detection methods. J. Histochem. Cytochem. 47(1):99-112 (1999).

Schöfer, C. et al. Signal amplification at the ultrastructural level using biotinylated tyramides and immunogold detection. Histochem Cell Biol. 108:313-319 (1997).

Stanarius, A. et al. Neuronal nitric oxide synthase in the vasculature of the rat brain: an immunocytochemical study using the tyramide signal amplification technique. J. Neurocytol. 27:731-736 (1998).

Stanarius, A. et al. Tyramide signal amplification in brain immunocytochemistry: adaptation to electron microscopy. J. Neuroscience Methods 88:55-61 (1999).

Angeletti, M. et al. Novel tyramide-based tyrosinase assay for the detection of melanoma cells in cytological preparations. Diagnostic Cytopathology 31(1):33-37 (2004).

de Haas, R.R. et al. The use of peroxidase-mediated deposition of biotin-tyramide in combination with time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization. J.Histochem. Cytochem. 44(10):1091-1099, 1996.

Fredricks, D. N. et al. Rhinosporidium seeberi: A Human Pathogen from a Novel Group of Aquatic Protistan Parasites. Emerg Infect Dis. 6(3):273-82.

Kressel, M. Tyramide amplification allows anterograde tracing by horseradish peroxidase-conjugated lectins in conjunction with simultaneous immunohistochemistry. J. Histochem. Cytochem. 46(4):527-533 (1998).

Kressel, M. and Radespiel-Troger, M. Anterograde tracing and immunohistochemical characterization of potentially mechanosensitive vagal afferents in the esophagus. J. Comp. Neurol. 412:161-172 (1999).

Kubota, K. et al. Visualization of mcr mRNA in a methanogen by fluorescence in situ hybridization with an oligonucleotide probe and two-pass tyramide signal amplification (two-pass TSA–FISH). J. Microbiol. Methods 66(3):521-528 (2006).

Liu, G. et al. A quantitative evaluation of peroxidase inhibitors for tyramide signal amplification mediated cytochemistry and histochemistry. Histochem Cell Biol.126(2):283-291 (2006).

Moritoyo, T. at al. Detection of human T-lymphotropic virus type I p40^tax protein in cerebrospinal fluid cells from patients with human T-lymphotropic virus type I-associated myelopathy/tropical spastic paraparesis. J. NeuroVirology 5:241-248 (1999).

Ness, J.M. et al. Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection. J. Histochem. Cytochem. 51(8):981-987 (2003).

Nguyen, D.G. and Hildreth, J.E.K. Involvement of macrophage mannose receptor in the binding and transmission of HIV by macrophages. European Journal of Immunology 33(2):483-493 (2003)

Pernthaler, A. et al. Fluorescence In Situ Hybridization and Catalyzed Reporter Deposition for the Identification of Marine Bacteria. Appl. Environ. Microbiol. 68:3094-3101 (2002).

Roth, K. A. at al. Enzyme-based antigen localization and quantitation in cell and tissue samples (Midwestern assay). J. Histochem. Cytochem. 45:1629-1641(1997).

Schönhuber, W. et al. Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Appl. Environ. Microbiol. 63:3268-3273 (1997).

Schönhuber, W. et al. In Situ Identification of Cyanobacteria with Horseradish Peroxidase-Labeled, rRNA-Targeted Oligonucleotide Probes. Appl. Environ. Microbiol. 65:1259-1267 (1997).

Shindler, K.S., C.B. Latham, and K.A. Roth. bax Deficiency Prevents the Increased Cell Death Of Immature Neurons in bcl-x-Deficient Mice. J. Neurosci. 17:3112-3119 (1997).

Svetlova, M.P., et al. Focal sites of DNA repair synthesis in human chromosomes. Biochem. Biophys. Res. Comm. 257(2):378-383 (1999).

Truong, K., et al. Signal amplification of FISH for automated detection using image cytometry. Anal. Cell Pathol. 13:137-146 (1997).

Van heusden, J., et al. Fluorescein-labeled tyramide strongly enhances the detection of low bromodeoxyusridine incorporation levels. J. Histochem. Cytochem. 45(2):315-319 (1997).

van Tine, B.A. et al. In situ analysis of the transcriptional activity of integrated viral DNA using tyramide-FISH. Dev Biol. (Basel) 106:381-385 (2001).

West, N.J. et al. Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides. Microbiology 147:1731-1744(2001).

Woodbury, R.L., et al. Elevated HGF Levels in Sera from Breast Cancer Patients Detected Using a Protein Microarray ELISA. Journal of Proteome Research 1 (3):233-237 (2002).

Böni J. et al. Simple monitoring of antiretroviral therapy with a signal-amplification-boosted HIV-1 p24 antigen assay with heat-denatured plasma. AIDS 11:F47-52 (1997).

Goldschmidt, P.L. et al. Comparison of an amplified enzyme-linked immunosorbent assay with procedures based on molecular biology for assessing human Immunodeficiency Virus type I viral load. Clin. Diag. Lab. Immunol. 5:513-518 (1998).

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Also refer to Society for Neuroscience site

Speel, E.J.M. et al. Amplification Methods to Increase the Sensitivity of In Situ Hybridization: Play CARD(S). J.Histochem. Cytochem. 47(3):281-288 (1999).

Speel E.J. et al. Tyramide signal amplification for DNA and mRNA in situ hybridization. Methods Mol Biol.326:33-60 (2006).

Qian X. and Lloyd R.V. Recent developments in signal amplification methods for in situ hybridization. Diagn Mol Pathol. 12(1):1-13 (2003).

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5,196,306　　Method for the detection or quantification of an analyte using an analyte dependent enzyme activation system.
5,583,001　　Method for detection or quantitation of an analyte using an analyte dependent enzyme activation system
5,731,158　　Catalyzed Reporter Deposition.
5,863,748　　p-Hydroxycinnamoyl-containing substrates for an analyte dependent enzyme activation system

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TSA増感システムとは？	シグナル増幅の基本原理	TSA増感システムを適用できる検出系
TSA増感システムの利点	TSAとin situハイブリダイザーション（ISH）	TSAとImmunohistochemistry