EnVision用蛍光色素・試薬・アッセイ別 フィルター・ミラー選択案内
| 蛍光色素・ 試薬・ アッセイ名 |
極大吸収波長(nm) | 極大放射波長(nm) | Excitation Filter | Optical Module/Mirror | Emission Filter | 備考 | |
| Xcite 1 PMT model |
HTS 2 PMTs model |
||||||
| Alamar Blue | NA | NA | 531nm/25nm (2100-5050) or 540nm/8nm (2100-5420) or 544nm/15nm (2100-5230) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
590nm/20nm (2100-5330) |
||
| Alexa Fluor - 488 | 495 | 519 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| Alexa Fluor - 555 | 553 | 568 | 540nm/8nm (2100-5420) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
572nm/7.5nm (2100-5080) or 580nm/10nm (2100-5610) |
||
| AMC | NA | NA | 380nm/10nm (2100-5390) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
460nm/25nm (2100-5130) |
MCA標識ペプチドを基質とした酵素反応の産物 | |
| Amplex Red | See Resorufin | ||||||
| ANS | 375 | 455 | 355nm/40nm (2100-5030) or 380nm/10nm (2100-5390) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
450nm/10nm (2100-5260) or 460nm/25nm (2100-5130) |
||
| BCECF | 503 | 528 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) | |
| BFP**** | 380 | 440 | 380nm/10nm (2100-5390) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
450nm/10nm (2100-5260) |
||
| BODIPY FL | 505 | 513 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| BODIPY TMR | 542 | 574 | 531nm/25nm (2100-5050) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
595nm/60nm (2100-5530) or 579nm/25nm (2100-5600) |
BODIPY TMR label (2100-8190) |
|
| Calcein | 494 | 517 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| CFP**** | 433 | 475 | 430nm/8nm (2100-5250) or 430nm/24nm (2100-5840) |
CFP/YFP / Dichroic 450_515nm (2100-4310)* |
470nm/24nm (2100-5850) or 486nm/10nm (2100-5270) |
||
| CFP-YFP FRET**** | 433 | 475, 527 | 430nm/8nm (2100-5250) or 430nm/24nm (2100-5840) |
CFP/YFP / Dichroic 450-515nm (2100-4310)* |
CFP/YFP Dual / Dichroic 450-505nm (2100-4320)** or CFP/YFP / Dichroic 450-515nm (2100-4310)* |
470nm/24nm (2100-5850) and 535nm/30nm (2100-5870) |
|
| Cy2 | 489 | 506 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| Cy3 | 550 | 570 | 531nm/25nm (2100-5050) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
595nm/60nm (2100-5530) or 579nm/25nm (2100-5600) |
BODIPY TMR label (2100-8190) |
|
| Cy5 | 649 | 670 | 620nm/10nm (2100-5240) or 620nm/40nm (2100-5760) |
Cy5 / Dichroic 658nm (2100-4240)* |
685nm/35nm (2100-5770) |
Cy5 label (2100-8190) |
|
| CyQuant | 502 | 523 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
||
| DAPI | 358 | 461 | 355nm/40nm (2100-5030) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
460nm/25nm (2100-5130) |
||
| DELFIA*** | 340 | 615 | 340nm/60nm (2100-5010) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
615nm/8.5nm (2100-5090) |
||
| DsRed2**** | See RFP | ||||||
| FITC | 494 | 518 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| Fluo-3 | 506 | 526 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| Fluo-4 | 494 | 516 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| Fura-2 | NA | NA | 340nm/25nm (2100-5190) and 380nm/10nm (2100-5390) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
510nm/10nm (2100-5320) or 510nm/20nm (2100-5380) |
2波長励起1波長測定のアッセイ、細胞内Ca2+測定 | |
| GeneBLAzer | 409 | 447, 520 | 400nm/25nm (2100-5370) |
Beta Lactamase / Dichroic 425nm (2100-4270)* |
Beta Lactamase Dual / Dichroic 425/490nm (2100-4270)** or Beta Lactamase / Dichroic 425nm (2100-4270)* |
460nm/25nm (2100-5130) and 535nm/25nm (2100-5120) |
Beta Lactamase label (2100-8430) or Beta Lactamase Dual label (2100-8420) |
| GFP**** | 488 | 509 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) or 510nm/20nm (2100-5380) |
FITC label (2100-8010) |
|
| H2DCFDA | 504 | 525 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| Hoechst 33258 | 352 | 455 | 355nm/40nm (2100-5030) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
460nm/25nm (2100-5130) |
||
| Hoechst 33342 | 352 | 455 | 355nm/40nm (2100-5030) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
460nm/25nm (2100-5130) |
||
| IQ Assay | 569 | 592 | 531nm/25nm (2100-5050) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
595nm/60nm (2100-5530) or 590nm/20nm (2100-5330) |
BODIPY TMR label (2100-8190) |
|
| LANCE*** | 330 | 615, 665 | 330nm/75nm (2100-5060) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
LANCE/DELFIA Dual / Dichroic 400/630nm LANCE/DELFIA / Dichroic 400nm |
615nm/8.5nm (2100-5090) and 665nm/7.5nm (2100-5110) |
|
| LanthaScreen*** | NA | NA | 340nm/60nm (2100-5010) or 330nm/75nm (2100-5060) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
495m/10nm (2100-5890) and 520nm/8nm (2100-5480) |
||
| MCA | See AMC | ||||||
| MOCAc/Dnp | 328 | 393 | 320nm/14nm (2100-5740) |
Dichroic 385 / Dichroic 385nm (2100-4180)* |
400nm/25nm (2100-5370) |
||
| monobromobimane (mBBr) | 394 | 490 | 400nm/25nm (2100-5370) |
Beta Lactamase / Dichroic 425nm (2100-4270)* |
510nm/20nm (2100-5380) or 515nm/30nm (2100-5300) |
||
| NADH | 340 | 445 | 340nm/14nm (2100-5750) or 340nm/25nm (2100-5190) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
450nm/10nm (2100-5260) or 460nm/25nm (2100-5130) |
||
| OliGreen | 502 | 522 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| PicoGreen | 502 | 522 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| Resorufin (Amplex Red) | 571 | 584 | 531nm/25nm (2100-5050) or 540nm/8nm (2100-5420) or 544nm/15nm (2100-5230) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
590nm/20nm (2100-5330) |
BODIPY TMR label (2100-8190) |
|
| RFP (DsRed2)**** | 558 | 583 | 531nm/25nm (2100-5050) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
595nm/60nm (2100-5530) |
BODIPY TMR label (2100-8190) |
|
| Rhodamine | 550 | 573 | 531nm/25nm (2100-5050) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
595nm/60nm (2100-5530) or 579nm/25nm (2100-5600) |
BODIPY TMR label (2100-8190) |
|
| Rhodamine 110 (R110) | 496 | 520 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| RiboGreen | 502 | 522 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| SYBR Green I | 498 | 522 | 485nm/14nm (2100-5020) |
FITC / Dichroic 505nm (2100-4030)* |
535nm/25nm (2100-5120) |
FITC label (2100-8010) |
|
| TAMRA | 542 | 568 | 531nm/25nm (2100-5050) |
BODIPY TMR / Dichroic 555nm (2100-4120)* |
595nm/60nm (2100-5530) or 579nm/25nm (2100-5600) |
BODIPY TMR label (2100-8190) |
|
| Texas-Red | 593 | 612 | 555nm/38nm (2100-5670) |
BS50 / Beam Splitter 50% (2100-4010) |
615nm/8.5nm (2100-5090) or 630nm/8nm (2100-5490) |
||
| Thioflavin T (ThT) | 444 | 490 | 430nm/24nm (2100-5840) or 440nm/8nm (2100-5660) |
CFP/YFP / Dichroic 450_515nm (2100-4310)* |
492nm/8nm (2100-5220) or 500nm/20nm (2100-5860) |
||
| Tryptophan | NA | NA | 280nm/10nm (2100-5350) |
BS50 / Beam Splitter 50% (2100-4010) |
340nm/14nm (2100-5750) or 355nm/40nm (2100-5030) |
||
| Umbelliferone | 360 | 449 | 355nm/40nm (2100-5030) |
LANCE/DELFIA / Dichroic 400nm (2100-4170)* |
460nm/25nm (2100-5130) |
Umbelliferone label (2100-8100) |
|
| YFP**** | 513 | 527 | 500nm/20nm (2100-5860) |
FITC / Dichroic 505nm (2100-4030)* |
530nm/10nm (2100-5280) or 535nm/30nm (2100-5870) |
||
*：感度は落ちるが[BS50 / Beam Splitter 50% (2100-4010)]も使用可能
**：感度は落ちるが[General Dual / BS50/BS50 (2100-4050)]も使用可能
***：TRFレーザー使用時はレーザー用ミラーを使用してください（要問合せ）
****：GFP関連の蛍光色素は、各社から多くの誘導体・変異体が販売されています。似たような名称でも蛍光特性が全く異なる場合がありますので、ご注意ください。
記載の蛍光色素名・試薬名・商品名は、各社の商標または登録商標です。
