TopCount Topics Application Notes Table of Contents
| TopCount Topics Application Notes Table of Contents | |
| 001. | Counting Non-Aqueous Samples in the TopCount Microplate Scintillation Counter Solid phase IRMA, efficiencies in PicoPlates, organic samples |
| 002. | Solid Scintillation Counting LumaPlates™ : efficiencies, crosstalk, color quench correction, 51Cr and 125I |
| 003. | Theory of TopCount Operation Basic scintillation theory, single-PMT time resolved pulse discrimination, efficiency and background data, fast vs. slow scintillators |
| 004. | Scintillation Proximity Assay on the TopCount Microplate Scintillation Counter SPA: results from RIA kits on TopCount® vs. LSC; color quench correction |
| 005. | Counting Aqueous Samples With the TopCount Microplate Scintillation Counter Aqueous counting: efficiencies, chemical quench correction, DPM recoveries, sample load vs. efficiency in MicroScint cocktails |
| 006. | TopCount Tandem Processing Using Automated Spreadsheets Spreadsheet macros and tandem processing for Lotus 123 and Quattro Pro |
| 007. | Solid-Phase RIA in Microplates RIA and IRMA in-plate binding, streptavidin, TopCount® vs. Gamma Counter, polystyrene resistance to MicroScint cocktails |
| 008. | Counting 51Cr Released in Cytotoxicity Assays Cytotoxicity (CML) assays, 51Cr, TopCount® vs. Gamma results, LumaPlates |
| 009. | Cell Proliferation Assays 3H thymidine uptake assay, TopCount® vs. LSC, UniFilter™, MicroMate 196™ |
| 010. | Two Methods of Harvesting Receptor Binding Assays Ligand-receptor binding, MicroMate 196™, Unifilter™, Brandel harvester |
| 011. | Direct Counting of Millipore® MultiScreen® Filtration Plates Cellular receptor binding, cell proliferation, enzyme inhibition and kinase assays, counting efficiencies |
| 012 | Biological Applications of Microplate Scintillation Counting General overview of cell proliferation, receptor binding, and in-plate binding assays, comparisons with LSC |
| 013. | Crosstalk Plate layout, 3H, 125I, 14C, 32P, chemiluminescence in a black plate |
| 014. | The Measurement of Luminescence Signal linearity and detection limits on TopCount, Promega (Luciferase), Lumigen (LumiPhos), Amersham (ECL, luminol), etc. |
| 015. | Quench and Quench Correction Basic theory of quench and correction, protocols for chemical and color quench correction curves on TopCount using UniFilter, LumaPlate, PicoPlate, OptiPlate |
| 016. | ViewPlates : Methodology for Counting Isotopic and Luminescent Labels for Use in Adherent Cell Assays Summary table of optimal counting parameters for ViewPlates on TopCount, efficiency and % optical crosstalk, 3 H, 14 C, 45 Ca, 32 P, 125 I, luminescence |
| 017. | Reporter Gene Assays Reporter gene, enzyme activity measurement assays, decay macro for short lived glow-type signals like Promega Luciferase, LumiGal |
| 018. | Counting Radioisotopic and Luminescent Labels on Filters and Membranes With the FlexiFilter Plate FlexiFilter for use in radioactive and non-radioactive detection, recommended try and collimator combinations, kinase assay, dot blot, cell proliferation |
| 019. | Quench Correction in Scintillation Proximity Assays Protocol for color quench correction for SPA |
| 020. | Nucleic Acid Quantification by Chemiluminescence Assay of Polymerase Chain Reaction Products Quantitative PCR, streptavidin coated plates, xanthine oxidase/ luminol |
| 021. | High Throughput Screening for cAMP Formation by Scintillation Proximity Radioimmunoassay Detecting cAMP by competitive SPA, 12-detectors, MultiPROBE |
| 022. | Determination of Neutrophil Activation by Chemiluminescence Using the Packard TopCount Microplate Scintillation and Luminescence Counter Low activity luminescent signal with 15 minute signal stability, LitePlate |
| 023. | High Throughput Luminescence Assays Using Six Detectors Multiple detector uniformity and linearity, LucLite and CytoLite |
| 024. | Cerenkov Counting Performance on the TopCount Microplate Scintillation and Luminescence Counter Counting performance on TopCount for linearity, volume effects, efficiency, background, and crosstalk, liquid and filters, microccentrifuge tube carriers |
| 025. | Automated Recording of Luciferase-Reported Gene Transcription in Living Seedlings and Fruit Flies Circadian rhythm, reporter gene assays, luciferase |
| 026. | Implementation of a Robotics system for High Throughput Screening Utilizing Glow Luminescence Cell based assay and compound distribution, MultiPROBE and Zymark integration |
| 027. | Use of TopCount for Radioactivity Determination in Wipe Test Use of the PicoPlate-24 and filter paper for wipe tests |
| 028. | Dual Label DPM on the TopCount Constant quench dual label DPM using calculations in custom cells |
| 029. | High Efficiency Count Mode for SPA and Cytostar-T Assays HECM using single pulse and lower discrimination adjustments |
| 030. | Optimization of 33P Scintillation Proximity Assays Using Cesium Chloride Bead Suspension Flotation method for SPA, reduces quench, higher efficiency |
| 031. | 33P Counting Performance on the TopCount and TopCount NXT Microplate Scintillation and Luminescence Counters Proves counting of 33P on the TopCount, and discusses optimization |
| 032. | Radioimmunoprecipitation Assay for Antibodies to Glutamic Acid Decarboxylase and Other Autoantigens in Microplates Unique application for UniFilter |
| AN4001-TC. | This application note was replaced with AN4003-TC. |
| AN4002-TC. | Calibration, Normalization and IPA of the TopCount NXT Serves as a supplement to the TopCount NXT Operation Manual |
| AN4003-TC. | Free Fat Cell Assay Validates MicroScint E and TopCount for in-plate lipid extraction and counting |
| AN004-TC. | Using the TopCount Microplate Scintillation and Luminescence Counter and Deep-Well LumaPlate Microplates in Combination with Micro Separation Techniques for Metabolic Studies Proves that TopCount/ LumaPlate is most sensitive for HPLC fraction counting |